Relative Stability of Pertussis Vaccine Preserved with Merthiolate, Benzethonium Chloride, or the Parabens1

Relative Stability of Pertussis Vaccine Preserved with Merthiolate, Benzethonium Chloride, or the Parabens.

When stored at 4 C, or heated at 22 or 35 C followed by storage at 4 C, the potency of pertussis vaccines preserved with Merthiolate was more stable than the potency of vaccines preserved with benzethonium chloride or the parabens (methyl- and propyl-p-hydroxybenzoate). Without preservative, potency was more stable than in the presence of benzethonium chloride or the parabens, but less stable t...

Identification of benzethonium chloride in commercial grapefruit seed extracts.

Commercial grapefruit seed extracts (GSE) were extracted with chloroform. The solvent was evaporated, and the resulting solid was subsequently analyzed by high-performance liquid chromatography, electrospray ionization mass spectrometry, nuclear magnetic resonance (NMR) spectroscopy, and elemental analysis (by proton-induced X-ray emission [PIXE] analysis). The main constituent was identified a...

Benzethonium chloride method for proteins adapted to centrifugal analysis.

A benzethonium chloride method for measuring protein concentrations in several types of body fluids has been adapted to the Cobas Bio centrifugal analyzer. The ability of this analyzer to add two reagents stepwise, measuring absorbances after each addition, is essential for proper blanking of urine specimens. The method has excellent curve stability and precision and yields results that correla...

Has the change to acellular pertussis vaccine improved or worsened pertussis control?

Pertussis Vaccine Effectiveness in the Setting of Pertactin-Deficient Pertussis.

BACKGROUND In the United States, the proportion of Bordetella pertussis isolates lacking pertactin, a component of acellular pertussis vaccines, increased from 14% in 2010 to 85% in 2012. The impact on vaccine effectiveness (VE) is unknown. METHODS We conducted 2 matched case-control evaluations in Vermont to assess VE of the 5-dose diphtheria, tetanus, and acellular pertussis vaccine (DTaP) ...